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KMID : 0389619960040040125
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BioForum
1996 Volume.4 No. 4 p.125 ~ p.136
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Progress and Prospect of Korea Rice Genome Research
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Eun Moo-Young
Cho Yong-Gu
Yi Bu-Young
Nahm Baek-Hie
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Abstract
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The Korea Rice Genome Research Program (KRGRP) was launched in September 1994 as a nationwide project in which many researchers firm universities and institutes are participating. The prograr. has focused on the constrwwn of molecular map of rice, map-based cloning, molecular breeding, in vitro selection ´of agronomic characters, cDNA sequencing and data-base construction.
For the breeding of mapping population, a ´MG´ recombinant inbred population which consists of 164 F13 lines has been developed from a crass between Tongil type variety Milyang 23 and Japonica type Gihobyeo by single seed descent With this population, a RFLP framework map has been constructed, and integration of morphological markers, isozyme loci, microsatellites, AFLPs, and new cDNA markers are being made for a highly saturated comprehensive map. A total of 208 RFLP markers, 205 AFLP markers, 65 microsatellites and 4 isozyme loci were integrated into the MG map. All marker types were well distributed throughout the 12 chromosomes. The intimated map covered 1,784 cM, and had an average interval size of 4.5 cM on the framework map, or 3.8 cM overall.
The existing MG map is a comerstone of the Korean Rice Genorne Research Program (KRGRP). It will be continuously refined through the addition of partially-sequenced cDNA markers derived from an immature seed cDNA library developed in Korea, and microsatellite markers developed at Comell. The framework map is being used for analyzing quantitative traits kxci(QIU) of agronomic characteristics and some physico-chenical properties related to rice quality. The number of significant QTLs affecting each trait ranged from one to five, and total 38 QTLs were detected in 17 traits. The percentage of variance explained by each QTL ranged firm 5.6 to 66.9%. The FstI-2 and two RFLP markers, RG109 and RG220, were linked most tightly at a distance less than 1 cM with the sd-1 gene on chromosome 1.
From the random sequencing of cDNA clones of immature seed cDNA library, about 3,500 cDNA clones were partially sequenced and analyzed according to homology. The Korea Rice Genome W W W and a mirror W W W sites for RiceGenes were developed and managed at NIAST, RDA and Myongji University. For the collection and organization of the data, the RiceMac database, a Macintosh version of RiceGenes was developed. The personal database is available at anonymous ftp site of Korea Rice Genome Server and AGIS, NAL. USDA. The CD-ROM version of RiceMac 4.3.1, is available upon written request
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